Confocal Raman Microscopy by Jan Toporski Thomas Dieing & Olaf Hollricher

Author:Jan Toporski, Thomas Dieing & Olaf Hollricher
Language: eng
Format: epub
Publisher: Springer International Publishing, Cham

Figure 14.1 demonstrates that confocal Raman distribution images are in perfect agreement with the images of the immunochemically stained tissue, clearly visualizing distribution of key cellular components and compartments. Additionally, AFM data complement Raman images with information about the tissue topography and compressibility.

In PART II (Sect. 14.3), the potential of Raman imaging of single cells is discussed. With Raman microscopy we can detect small biochemical changes and their distribution at the sub-cellular level [9]. Investigations of cells allow tracking of changes under the impact of various factors, e.g. monitoring the uptake of drugs, nanoparticles and bioactive compounds, as well as non-chemical stressors [10–14]. Raman measurements with spatial resolution 300nm (limited by Rayleigh criterion) allow for detection and characterization of such small structures as nucleolus, nucleoli, mitochondria, lipid droplets (LDs; or lipid bodies ) or introduced nanoparticles [15–18].

In cell biology most commonly used methods are electron microscopy and fluorescence microscopy. Each of these techniques requires a specific sample preparation, thus modifying sample composition and changing its physiological conditions. With Raman microscopy, changes in the cells during the cell cycle, cell death, drug-cell interactions, proliferation and differentiation can be successfully studied avoiding the above mentioned modifications [19, 20].

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